DNA barcoding adalah metode identifikasi spesies menggunakan urutan gen pendek yang terstandarisasi. Metode ini pertama kali diusulkan oleh Hebert et al. (2003) dan telah menjadi salah satu metode identifikasi spesies yang paling banyak digunakan dalam penelitian taksonomi.
DNA barcoding menggunakan urutan gen pendek yang disebut DNA barcode. DNA barcode biasanya berasal dari gen mitokondria, yaitu organel yang menghasilkan energi untuk sel. Gen mitokondria umumnya memiliki variasi urutan yang tinggi antarspesies, tetapi relatif konstan dalam satu spesies.
Untuk melakukan DNA barcoding, sampel DNA dari spesimen yang ingin diidentifikasi diisolasi dan disekuensikan. Hasil sekuen kemudian dibandingkan dengan database DNA barcode yang telah tersedia. Jika urutan DNA spesimen cocok dengan urutan DNA di dalam database, maka spesimen tersebut dapat diidentifikasi ke tingkat spesies.
DNA barcoding memiliki beberapa keunggulan dibandingkan dengan metode identifikasi spesies lainnya, antara lain:
DNA barcoding memiliki berbagai aplikasi, antara lain:
DNA barcoding adalah metode identifikasi spesies yang cepat, akurat, dan dapat digunakan untuk berbagai jenis organisme. Metode ini telah menjadi salah satu metode identifikasi spesies yang paling banyak digunakan dalam penelitian taksonomi.
WebApa itu DNA Barcoding? Teknik ini digunakan untuk mengurutkan basa DNA yang sangat berbeda-beda antarspesies sehingga dapat dipakai sebagai penanda suatu spesies. Dr Hawis Maddupa, dosen IPB University sekaligus Kepala Laboratorium Biodiversitas dan Biosistematika Kelautan menjelaskan teknik DNA barcoding dilakukan. WebHebert et al. (2003) menyatakan bahwa DNA Barcoding adalah urutan sekuen pendek DNA yang telah terstandarisasi untuk identifikasi spesies hewan secara tepat, cepat, dan akurat, sedangkan P etit &
DNA Barcoding: Konsep dan Aplikasinya | GeneCraft Labs Articles – Source: GeneCraft Labs
What is DNA Barcoding? – iBOL – Source: What is DNA Barcoding? – iBOL
DNA Barcoding Service – biome-id – Source: biome-id
Apa Itu Dna Barcoding, DNA Barcoding, 4.94 MB, 03:36, 77,037, Agriculture and Agri-Food Canada, 2014-12-08T14:45:26.000000Z, 2, DNA Barcoding: Konsep dan Aplikasinya | GeneCraft Labs Articles, GeneCraft Labs, 302 x 685, jpg, , 3, apa-itu-dna-barcoding
Apa Itu Dna Barcoding.
DNA barcoding uses a very short segment of DNA to identify organisms at the genetic level. Because no two species have the same DNA, this segment acts like the black stripes of the Universal Product Code (UPC) to create a unique identification sequence. Once the DNA barcodes are created, researchers then record them in databases to produce an accurate catalogue of life on earth.
Agriculture and Agri-Food Canada is taking the lead on creating and adding thousands of DNA barcodes to national and international databases. The benefits to the Canadian agricultural sector are many. Learn how this work helps to protect our crops and agricultural exports.
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Full Video Transcript:
Narrator: DNA is a molecule that encodes the genetic instructions of an organism, and it is unique to each species. DNA barcoding is a method that uses an organisms DNA to identify it as belonging to a certain species.Narrator: In Canada, our scientists are at the leading edge of DNA barcoding. At Agriculture and Agri-Food Canada, we have been identifying thousands of good and bad organisms, and registering their DNA barcodes to international databanks. The benefits of this work on DNA barcoding are huge for our agricultural crops, livestock and trade.
Dr. Levesque: I work in mycology. And there are many cases where the organism would look the same in culture. It would look the same under the microscope. And we do need DNA sequencing to figure out if they are different species or not. Most Canadians don’t realize it but identifying organisms is part of daily life. Well you go to a supermarket and you want to make sure that there’re no pathogens in there that we did not import plant quarantined organisms in the food we’re eating.
Dr. Levesque: So it is part of the daily life. And we don’t see it.
Tom Graefenhan: With the Canadian Food Inspection Agency we explore state of the art technology, such as next generation sequencing and metagenomics for the detection of pathogens in cereals and other grains. Accurate and reliable identification of these microorganisms is paramount. DNA barcode data sets supports the information provided on phyto-sanitary certificates and statements of assurance for grain shipments. These certificates and statements secure free market access for Canadian grain exports worth billions of dollars every year.
Dr. André Levesque: The challenges that DNA barcoding can resolve are really numerous. For my lab and our group here we are really focusing on quarantine and invasive species. One of these organisms can really cause hundreds of millions of dollars in trade disruptions. So we are trying to prevent the introduction. And we are trying to develop tools to make sure that what we export is clean.
Tom Graefenhan: A recent example where DNA barcoding played an important role was a shipment of pulses to South America. By linking newly acquired DNA baseline data with specimen and other historical information we were able to quickly confirm the absence of the pathogen in Canada, assuring compliance of the multi-million dollar cargo.
Dr. André Levesque: The future is very promising for this kind of technology. The technology now is such that we’re making if more available and easier to use by a wider range of people.
DNA Barcoding: Konsep dan Aplikasinya | GeneCraft Labs Articles
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25 › menentukanMenentukan Biodiversiti Hewan Air dengan DNA Barcoding dan …
Sapto memiliki latar belakang penelitian di bidang molekuler dan disini Sapto akan menjelaskan apa itu DNA Barcoding dan metabarcoding yang akan menguak keanekaragaman spesies ikan. Melansir dari FishBase, sebanyak 3.701 spesies ikan laut, namun faktanya, sebanyak 8.500 spesies ikan laut di Samudera Hindia. .
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NA_barcodingDNA barcoding – Wikipedia
DNA barcoding is a method of species identification using a short section of DNA from a specific gene or genes. The premise of DNA barcoding is that by comparison with a reference library of such DNA sections (also called “sequences”), an individual sequence can be used to uniquely identify an organism to species, just as a supermarket scanner uses the familiar black stripes of the UPC barcode , .
ora › 328479Riset dengan DNA Barcoding di Indonesia masih Bisa Dihitung Jari
Apa itu DNA Barcoding? Teknik ini digunakan untuk mengurutkan basa DNA yang sangat berbeda-beda antarspesies sehingga dapat dipakai sebagai penanda suatu spesies. .
-inovasi-penelitianDNA Barcoding Inovasi Penelitian Taksonomi – Warung Sains …
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codingDNA Barcoding: Konsep dan Aplikasinya – GeneCraft Labs
DNA barcoding merupakan suatu sistem yang dirancang untuk memudahkan identifikasi dan autentifikasi makhluk hidup secara cepat dan akurat karena menggunakan sekuen gen pendek dan telah terstandarisasi. Panjang urutan gen pada DNA barcoding berkisar antara 400-600 pasang basa. .
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